abstract：:The study of the light-harvesting complex II (LHC-II) phosphatase activity has been difficult due to the membrane association of its substrate. Thylakoid membranes labeled with [γ-(32)P]ATP were incubated with chymotrypsin, releasing phosphopeptides which served as labeled substrates for LHC-II phosphatase. Utilizing ...

journal_title：Photosynthesis research

authors： Hammer MF,Sarath G,Osterman JC,Markwell J

更新日期：1995-05-01 00:00:00

abstract：:The pigment composition of the light-harvesting complexes of Photosystem II (LHC II) has been determined for lettuce (Lactuca sativa). In common with other members of the composite, the photosynthetic tissues of this species may contain large amounts of the carotenoid lactucaxanthin (ε, ε-carotene-3,3'-diol) in additi...

journal_title：Photosynthesis research

authors： Phillip D,Young AJ

更新日期：1995-03-01 00:00:00

abstract：:A rapid procedure has been developed for the isolation of the photosystem two reaction centre complex (PS II RC) from a double mutant of Chlamydomonas reinhardtii, F54-14, which lacks the Photosystem one complex and the chloroplast ATPase. Thylakoid membranes are solubilised with 1.5% (w/v) Triton X-100 and the PS II ...

journal_title：Photosynthesis research

authors： Alizadeh S,Nixon PJ,Telfer A,Barber J

更新日期：1995-02-01 00:00:00

abstract：:The protonophoric uncouplers carbonyl cyanide m-chlorophenylhydrazone (CCCP), 2,3,4,5,6-pentachlorophenol (PCP) and 4,5,6,7-tetrachloro-2-trifluoromethylbenzimidazole (TTFB) inhibited the Hill reaction with K3[Fe(CN)6] (but not with SiMo) in chloroplast and cyanobacterial membranes (the I50 values were approx. 1-2, 4-...

journal_title：Photosynthesis research

authors： Samuilov VD,Renger G,Paschenko VZ,Oleskin AV,Gusev MV,Gubanova ON,Vasil'ev SS,Barsky EL

更新日期：1995-01-01 00:00:00

abstract：:A simple approach to determine CO2/O2 specificity factor (τ) of ribulose 1,5-bisphosphate carboxylase/oxygenase is described. The assay measures the amount of CO2 fixation at varying [CO2]/[O2] ratios after complete consumption of ribulose 1,5-bisphosphate (RuBP). Carbon dioxide fixation catalyzed by the carboxylase w...

journal_title：Photosynthesis research

authors： Kostov RV,McFadden BA

更新日期：1995-01-01 00:00:00

abstract：:The interaction of PsaC with the core heterodimer of Photosystem 1 was studied in wild type Synechocystis sp. PCC 6803 and the site-directed mutant R561E of PsaB. The mutant reaction center was much less stable in urea and the functional reconstitution of the mutant core using PsaC was impaired. However, the extent of...

journal_title：Photosynthesis research

authors： Rodday SM,Schulz R,McLntosh L,Biggins J

更新日期：1994-12-01 00:00:00

abstract：:In this paper, we describe a protocol to obtain a site-directed mutants in thepsbA gene ofChlamydomonas reinhardtii, which overcomes several drawbacks of previous protocols, and makes it possible to generate a mutant within a month. Since the large size of the gene, and the presence of four large introns has made mole...

journal_title：Photosynthesis research

authors： Minagawa J,Crofts AR

更新日期：1994-11-01 00:00:00

abstract：:For maximal rates of CO2 assimilation in isolated intact spinach chloroplasts the generation of the adequate NADPH/ATP ratio is achieved either by cyclic electron flow around photosystem I or by linear electron transport to oxaloacetate, nitrite or oxygen (Mehler-reaction). The interrelationships between these poising...

journal_title：Photosynthesis research

authors： Backhausen JE,Kitzmann C,Scheibe R

更新日期：1994-10-01 00:00:00

abstract：:A study of the absorption and fluorescence characteristics of the D1/D2/cytb-559 reaction centre complex of Photosystem II has been carried out by gaussian decomposition of absorption spectra both at room temperature and 72 K and of the room temperature fluorescence spectrum. A five component fit was found in which th...

journal_title：Photosynthesis research

authors： Garlaschi FM,Zucchelli G,Giavazzi P,Jennings RC

更新日期：1994-09-01 00:00:00

abstract：:Recommendations are given for naming of genes coding for reaction center, antenna and electron transport proteins in green photosynthetic bacteria and heliobacteria. ...

journal_title：Photosynthesis research

authors： Bryant DA

更新日期：1994-07-01 00:00:00

abstract：:The dependence of chlorosome development on bacteriochlorophyll (BChl)c synthesis was studied with the phototrophic green bacteriumChloroflexus aurantiacus. By selecting defined culture conditions, three possibilities could be identified. Upon addition of 5-aminolevulinic acid, cells of resting cultures increased thei...

journal_title：Photosynthesis research

authors： Foidl M,Golecki JR,Oelze J

更新日期：1994-07-01 00:00:00

abstract：:Artificial aggregates of bacteriochlorophyllc (BChlc) were formed in an aqueous medium in the presence of a lipid, monogalactosyl diglyceride (MGDG), and the optical properties of those aggregates were studied by absorption and circular dichroism (CD) mainly. Four BChlc homologs, ([E,E]BChlc F, [P,E]BChlc F, [E,M]BChl...

journal_title：Photosynthesis research

authors： Uehara K,Mimuro M,Ozaki Y,Olson JM

更新日期：1994-07-01 00:00:00

abstract：:This personal perspective records research experiences in chemistry and biology at four German universities, two before and two after World War II. The research themes came from cytophysiology of green unicellular algae, in particular their photosynthesis. The function of inorganic ions in photosynthesis and dark resp...

journal_title：Photosynthesis research

authors： Pirson A

更新日期：1994-06-01 00:00:00

abstract：:We have used antibodies generated against synthetic peptides to determine the topology of the 43 kD chlorophyll a binding protein (CP 43) of Photosystem II. Based on the pattern of proteolytic fragments detected (on western blots) by peptide specific antibodies, a six transmembrane span topological model, with the ami...

journal_title：Photosynthesis research

authors： Sayre RT,Wrobel-Boerner EA

更新日期：1994-04-01 00:00:00

abstract：:Analysis of leaf-level photosynthetic responses of 39 tree species grown in elevated concentrations of atmospheric CO2 indicated an average photosynthetic enhancement of 44% when measured at the growth [CO2]. When photosynthesis was measured at a common ambient [CO2], photosynthesis of plants grown at elevated [CO2] w...

journal_title：Photosynthesis research

authors： Gunderson CA,Wullschleger SD

更新日期：1994-03-01 00:00:00

abstract：:A high CO2-requiring mutant of Synechocystis PCC6803 (G3) capable of Ci transport but unable to utilize the intracellular Ci pool for photosynthesis was constructed. A DNA clone of 6.1 kbp that transforms the G3 mutant to the wild-type phenotype was isolated from a Synechocystis PCC6803 genomic library. Complementatio...

journal_title：Photosynthesis research

authors： Ogawa T,Amichay D,Gurevitz M

更新日期：1994-02-01 00:00:00

abstract：:The factors governing quinone protein binding in the photosynthetic bacterium Rps. viridis are investigated via use of empirical intermolecular energy calculations using the GRID method (PJ Goodford (1985) J Med Chem 28: 849-854). A probe atom corresponding to a carbonyl oxygen atom is used to investigate regions exhi...

journal_title：Photosynthesis research

authors： O'Malley PJ,Braithwaite CJ

更新日期：1994-01-01 00:00:00

abstract：:The chlorina-f2 mutant of barley (Hordeum vulgare L.) contains no chlorophyll b in its light-harvesting antenna, whereas the chlorina-103 mutant contains approximately 10% of the chlorophyll b found in wild-type. The absolute chlorophyll antenna size for Photosystem-II in wild-type, chlorina-103 and chlorina-f2 mutant...

journal_title：Photosynthesis research

authors： Harrison MA,Nemson JA,Melis A

更新日期：1993-11-01 00:00:00

abstract：:The rate and extent of light activation of PEPC may be used as another criterion to distinguish C3 and C4 plants. Light stimulated phosphoenolypyruvate carboxylase (PEPC) in leaf discs of C4 plants, the activity being three times greater than that in the dark but stimulation of PEPC was limited about 30% over the dark...

journal_title：Photosynthesis research

authors： Rajagopalan AV,Devi MT,Raghavendra AS

更新日期：1993-10-01 00:00:00

abstract：:Triplet state electron paramagnetic resonance (EPR) experiments have been carried out at X-band on Rb. sphaeroides R-26 reaction centers that have been reconstituted with the carotenoid, spheroidene, and exchanged with 13(2)-OH-Zn-bacteriochlorophyll a and [3-vinyl]-13(2)-OH-bacteriochlorophyll a at the monomeric, 'ac...

journal_title：Photosynthesis research

authors： Frank HA,Chynwat V,Hartwich G,Meyer M,Katheder I,Scheer H

更新日期：1993-09-01 00:00:00

abstract：:Ten strains from a collection of mutants ofSynechocystis 6803 defective in Photosystem II (PS II) function were transformed with chromosomal DNA of wild-type and mutant cells. Cross hybridization data allowed to identify four groups of PS II-mutants. Highly efficient transformation was observed between different mutan...

journal_title：Photosynthesis research

authors： Ermakova SY,Elanskaya IV,Kallies KU,Weihe A,Börner T,Shestakov SV

更新日期：1993-08-01 00:00:00

abstract：:A proton electrochemical potential across the membranes of photosynthetic purple bacteria is established by a light-driven proton pump mechanism: the absorbed light in the reaction center initiates electron transfer which is coupled to the vectorial displacement of protons from the cytoplasm to the periplasm. The stoi...

journal_title：Photosynthesis research

authors： Maróti P

更新日期：1993-07-01 00:00:00

abstract：:Recently, a number of techniques, some of them relatively new and many often used in combination, have given a clearer picture of the dynamic role of electron transport in Photosystem I of photosynthesis and of coupled cyclic photophosphorylation. For example, the photoacoustic technique has detected cyclic electron t...

journal_title：Photosynthesis research

authors： Fork DC,Herbert SK

更新日期：1993-06-01 00:00:00

abstract：:Two-component regulatory systems that respond to changes in redox potential have recently been discovered in bacteria. 'Redox sensors' are defined as electron carriers which initiate control of gene expression upon oxidation or reduction. 'Redox response regulators' are defined as DNA-binding proteins which modify gen...

journal_title：Photosynthesis research

authors： Allen JF

更新日期：1993-05-01 00:00:00

abstract：:Inorganic phosphate participates in many fundamental processes within the plant cell. Its broad influence on plant metabolism is related to such key operations as metabolite transport, enzyme regulation and carbohydrate metabolism in general. This review discusses these topics with special emphasis on the role assigne...

journal_title：Photosynthesis research

authors： Iglesias AA,Plaxton WC,Podestá FE

更新日期：1993-03-01 00:00:00

abstract：:We have compared the properties of a mutant of barley lacking Photosystem I (viridis-zb (63) ) with the corresponding wild type using modulated fluorescence measurements. The mutant showed two unexpected characteristics. Firstly, there was a slow decline in the fluorescence signal in the light which was dependent on t...

journal_title：Photosynthesis research

authors： Juhler RK,Miller M,Simpson D,Cox RP

更新日期：1993-03-01 00:00:00

abstract：:In polyploid plants the photosynthetic rate per cell is correlated with the amount of DNA per cell. The photosynthetic rate per unit leaf area is the product of the rate per cell times the number of photosynthetic cells per unit area. Therefore, the photosynthetic rate per unit leaf area will increase if there is a le...

journal_title：Photosynthesis research

authors： Warner DA,Edwards GE

更新日期：1993-02-01 00:00:00

abstract：:This review describes the progress in our understanding of the structure of the Mn complex in Photosystem II over the last two decades. Emphasis is on the research from our laboratory, especially the results from X-ray absorption spectroscopy, low temperature electron paramagnetic resonance and electron spin echo enve...

journal_title：Photosynthesis research

authors： Klein MP,Sauer K,Yachandra VK

更新日期：1993-01-01 00:00:00

abstract：:Earlier mass spectrometric measurements, in which oxygen evolution was measured following short saturating light flashes, indicated that with a time resolution of about 30 s no form of bound water and/or an oxidation product exists up to the redox state S3 of the oxygen evolving center (R. Radmer and O. Ollinger, 1986...

journal_title：Photosynthesis research

authors： Bader KP,Renger G,Schmid GH

更新日期：1993-01-01 00:00:00

abstract：:A new binding site for anions which inhibit the water oxidizing complex (WOC) of Photosystem II in spinach has been identified. Anions which bind to this site inhibit the flash-induced S2/S0 catalase reaction (2H2O2→2H2O+O2) of the WOC by displacing hydrogen peroxide. Using a mass spectrometer and gas permeable membra...

journal_title：Photosynthesis research

authors： Mano J,Kawamoto K,Dismukes GC,Asada K

更新日期：1993-01-01 00:00:00

abstract：:The activity of ribulose-1,5-bisphosphate carboxylase/oxygenase fromEuglena gracilis decays steadily when exposed to agents that induce oxidative modification of cysteine residues (Cu(2+), benzofuroxan, disulfides, arsenite, oxidized ascorbate). Inactivation takes place with a concomitant loss of cysteine sulfhydryl g...

journal_title：Photosynthesis research

authors： García-Ferris C,Moreno J

更新日期：1993-01-01 00:00:00

abstract：:MOA-stilbene is known to be a specific inhibitor of the Qo site of mammalian cytochrome bc 1 complex. We show that it also binds to the chloroplast cytochrome bf complex. Binding to the reduced enzyme induces a red-shift of the Soret and visible absorption bands of the haems b. Steady state and single turnover experim...

journal_title：Photosynthesis research

authors： Rich PR,Madgwick SA,Brown S,von Jagow G,Brandt U

更新日期：1992-12-01 00:00:00

abstract：:A three-dimensional model of the core proteins D1 and D2, including the cofactors, that form the Photosystem II reaction centre of pea (Pisum sativum), has been generated. This model was built with a rule-based computer modelling system using the information from the crystal structures of the photosynthetic reaction c...

journal_title：Photosynthesis research

authors： Ruffle SV,Donnelly D,Blundell TL,Nugent JH

更新日期：1992-11-01 00:00:00

abstract：:There is diverse, yet controversial fossil evidence for the existence of photosynthesis 3500 million years ago. Among the most persuasive evidence is the stromatolites described from low grade metasedimentary rocks in Western Australia and South Africa. Based on the understanding of the paleobiology of stromatolites a...

journal_title：Photosynthesis research

authors： Awramik SM

更新日期：1992-08-01 00:00:00

abstract：:The Photosystem I reaction centre protein CP1, isolated from barley using polyacrylamide gel electrophoresis showed an EPR (Electron Paramgnetic Resonance) spectrum with the polarisation pattern AEEAAE, typical of the primary donor triplet state (3)P700, created via radical pair formation and recombination. (3)P700 co...

journal_title：Photosynthesis research

authors： Searle GF,Schaafsma TJ

更新日期：1992-06-01 00:00:00

abstract：:Bioenergetic properties of thylakoids from plants submitted to a water stress stress (watering stopped for 6-15 days) have been measured in two lupin genotypes characterized as resistant or susceptible to drought. This energy coupling was assessed by flow-force relationships relating the phosphorylation rate to the ma...

journal_title：Photosynthesis research

authors： Meyer S,Phung Nhu Hung S,Trémolières A,de Kouchkovsky Y

更新日期：1992-05-01 00:00:00

abstract：:This study was conducted to more clearly define the physiological effects of PS II herbicides on chloroplast thylakoid membrane activity and composition. Barley (Hordeum vulgare L. cv Boone) was grown in hydroponic culture at 20°C in a growth chamber with a light intensity of 500 μmole photons m(-2) s(-1). Atrazine (2...

journal_title：Photosynthesis research

authors： De la Torre WR,Burkey KO

更新日期：1992-04-01 00:00:00

abstract：:Since 1986, non-uniform photosynthesis over the leaf area that may be attributed to patchy stomatal closure, has been an important issue in stress physiology of photosynthesis. In this review, I first outline the gaseous environment within the intercellular spaces, because this is the most fundamental background of th...

journal_title：Photosynthesis research

authors： Terashima I

更新日期：1992-03-01 00:00:00

abstract：:To determine the fluorescence properties of cyanobacterial Photosystem I (PS I) in relatively intact systems, fluorescence emission from 20 to 295 K and polarization at 77 K have been measured from phycobilisomes-less thylakoids of Synechocystis sp. PCC 6803 and a mutant strain lacking Photosystem II (PS II). At 295 K...

journal_title：Photosynthesis research

authors： Wittmershaus BP,Woolf VM,Vermaas WF

更新日期：1992-02-01 00:00:00

abstract：:The binding characteristics and the inhibitory power of atrazine and DCMU towards uncoupled electron flow activity were studied in acyl lipid-depleted thylakoid membranes from atrazine-susceptible and-resistant biotypes of Solanum nigrum L. For this purpose, phospholipase A2 from Vipera russelli and the lipase from Rh...

journal_title：Photosynthesis research

authors： Siegenthaler PA,Mayor JP

更新日期：1992-01-01 00:00:00